Skip to main content
. 2021 Mar 26;4:421. doi: 10.1038/s42003-021-01900-0

Fig. 3. Reduced islet and serum serotonin in vitamin B6-deficient dams at GD 12.5 and 16.5, respectively.

Fig. 3

Representative ×20 immunofluorescent images of GD 12.5 pancreases from control and vitamin B6-deficient or VB6D dams (A). Corrected total cell fluorescence (CTCF) was used to calculate islet serotonin in the GD 12.5 pancreases (B). In (C), we used LC-HRMS to measure absolute islet serotonin levels. Relative serum serotonin levels in GD 12.5 (D) and 16.5 (E) control and VB6D dams were measured using LC-MS. We analyzed 40–50 islets per mouse (N = 3 mice) in panels (A) and (B), 6–10 mice in panel (C), and 5–7 mice in panels (D) and (E). Unpaired, one-sided, t-tests were performed in (B–E). *p ≤ 0.05; non-significant (ns) represents p values > 0.1.