Figure 5.
Ketone body supplementation did not influence the severity of CCl4-induced liver fibrosis. (A) Study design of the in vivo experiment. (B) Serum samples were collected at the end of the experiment, and the serum ALT was assessed. (C) Representative results from Western blot analyses of α-SMA expression and (D) qPCR analyses of α-SMA and Col1a2 gene expression. (E) Representative H&E, Sirius red, and Masson’s trichrome staining images of liver tissues. Scale bars: 0.1 mm. *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs. relative control. N = 5 in each group. Data are shown as mean ± SEM.