Table 5.
Intracellular marker expression of rabbit and human endothelial cells analyzed by flow cytometry.
| Marker/Passage | Cell Type | P0 | P1 | P2 | P3 | HUVECs (P3) |
|---|---|---|---|---|---|---|
| AcLDL | EPCs | 80.8 ± 10.8 | 92.8 ± 7.8 | 88.7 ± 13.9 | 92.2 ± 5.4 | 86.2 ± 1.0 |
| BEPCs | 99.3 ± 0.5 | 99.9 ± 0.2 | 98.8 ± 1.0 | 97.6 ± 0.9 ** | ||
| ALDH | EPCs | 84.9 ± 5.6 | 78.9 ± 10.3 | 80.4 ± 15.4 | 94.5 ± 5.0 | 80.0 ± 1.8 |
| BEPCs | 24.7 ± 4.9 | 46.1 ± 25.2 | 48.6 ± 40.4 | 25.3 ± 10.5 | ||
| Vimentin | EPCs | 81.3 ± 5.9 | 87.3 ± 7.7 | 89.6 ± 7.3 | 86.3 ± 9.0 | n/a |
| BEPCs | 99.3 ± 0.3 | 98.0 ± 1.5 | 99.4 ± 0.5 | 99.0 ± 0.7 | ||
| Desmin | EPCs | 55.1 ± 18.2 | 59.0 ± 17.9 | 68.3 ± 20.2 | 64.2 ± 14.0 | n/a |
| BEPCs | 89.5 ± 5.8 | 92.3 ± 6.0 | 94.8 ± 3.4 | 92.4 ± 5.4 | ||
| α-SMA | EPCs | 74.0 ± 5.9 | 85.6 ± 11.1 | 88.5 ± 4.9 | 82.5 ± 9.9 | n/a |
| BEPCs | 99.0 ± 0.3 | 96.9 ± 2.0 | 98.1 ± 1.1 | 92.7 ± 10.4 | ||
| vWF | EPCs | 98.0 ± 0.1 | 98.3 ± 0.8 | 99.4 ± 0.3 * | 99.8 ± 0.1 ** | 98.8 ± 1.5 |
| BEPCs | 97.4 ± 1.4 | 99.5 ± 0.3 ** | 99.7 ± 0.2 *** | 99.8 ± 0.1 *** | ||
| eNOS | EPCs | 94.8 ± 6.0 | 99.0 ± 0.3 | 99.4 ± 0.2 | 99.7 ± 0.1 | 98.9 ± 1.0 |
| BEPCs | 99.8 ± 0.1 | 99.9 ± 0.1 | 99.9 ± 0.1 | 99.9 ± 0.1 |
The data are expressed as the mean ± SD; P0—initial culture, P1—first passage, P2—second passage, P3—third passage; AcLDL—acetylated low-density lipoprotein; ALDH—aldehyde dehydrogenase; α-SMA—α-smooth muscle actin; vWF—von Willebrand factor; eNOS—endothelial nitric oxide synthase; *—difference is statistically significant at p < 0.05; **—difference is statistically significant at p < 0.01.; ***—difference is statistically significant at p < 0.001 in comparison to P0; n/a—not analyzed.