Table 3.
Results of the antioxidant DPPH assay using Leg1/Leg2 and single amino acids in a concentration range of 15.6–1000 µM. Ascorbic acid was used as positive control. The mean ± SD of triplicates of the antioxidative activity is displayed.
| Concentration [µM] | Antioxidant Activity (%) | ||||||
|---|---|---|---|---|---|---|---|
| 1000 | 500 | 250 | 125 | 62.5 | 31.3 | 15.6 | |
| Leg1 (RIKTVTSFDLPALRFLKL) | 29.3 ± 3.7 | 6.0 ± 8.2 | 0.30 ± 0.5 | - 1 | - | - | - |
| Leg2 (RIKTVTSFDLPALRWLKL) | 91.5 ± 8.3 | 72.2 ± 12.8 | 46.2 ± 16.6 | 20.9 ± 22.6 | 26.7 ± 1.3 | 17.7 ± 2.3 | 5.3 ± 3.3 |
| Tyrosine | 50.3 ± 6.0 | 52.4 ± 4.5 | 42.3 ± 11.6 | 31.2 ± 4.5 | 40.1 ± 7.5 | 10.4 ± 12.2 | - |
| Histidine | 34.5 ± 9.1 | 32.8 ± 7.3 | 30.0 ± 3.2 | 17.9 ± 11.9 | 19.1 ± 3.7 | 6.1 ± 4.0 | 0.2 ± 6.4 |
| Cysteine | 93.6 ± 1.9 | 82.6 ± 8.9 | 95.7 ± 1.5 | 94.1 ± 2.2 | 90.2 ± 8.8 | 88.5 ± 7.2 | 72.8 ± 11.6 |
| Tryptophan | 60.8 ± 11.1 | 38.6 ± 2.5 | 22.8 ± 4.4 | 15.2 ± 7.9 | 17.9 ± 5.8 | 19.5 ± 5.6 | 17.6 ± 5.7 |
| Proline | 28.7 ± 9.4 | 25.6 ± 10.9 | 23.3 ± 5.9 | 19.0 ± 11.6 | 8.5 ± 8.5 | - | - |
| Phenylalanine | 29.0 ± 6.2 | 20.5 ± 3.9 | 18.1 ± 8.4 | 19.3 ± 14.2 | 10.6 ± 6.4 | - | - |
| Ascorbic acid | 91.2 ± 2.4 | 82.2 ± 8.2 | 74.3 ± 13.2 | 79.2 ± 15.4 | 70.1 ± 4.2 | 71.9 ± 6.9 | 58.9 ± 1.9 |
1 (-) no antioxidative activity detected.