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. 2021 Mar 11;13(3):459. doi: 10.3390/v13030459

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sequence logo of the 10 bases upstream and the 10 bases downstream of the protospacers. The -GA(A)- sequence in the 10 bases downstream is likely the PAM sequence and is downstream of 17 of the 18 protospacers.