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. 2021 Mar 9;10(3):279. doi: 10.3390/antibiotics10030279

Figure 1.

Figure 1

Tailspike protein (TSP) overexpression and activity assay. (A) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of Ni-NTA His∙Bind® Resin affinity column purified TSP. The protein sample was run on a reducing 10% gel. Lanes: M, molecular weight markers; TSP, purified protein. (B) TSP activity against its host, Ab-54149. Serial dilutions of TSP were dropped on the host strain and 20 mM PBS was used as the negative control.