Figure 4.

Reduction of Ab-54149 biofilm thickness by tailspike protein (TSP). Representative confocal microscope images of Ab-54149 showing biofilm architecture established after 48 h of incubation at 37 °C (A) and following 4 h treatment with 100 ng TSP (B) and quantification of both biofilm thicknesses (C). The bar graph represents the mean ± SD. Statistical significance was analyzed by a two-tailed Student’s t-test (p < 0.05). The biofilm spatial characteristic was determined by ISA confocal analysis software.