Figure 9.

Therapeutic efficacy of depolymerase in zebrafish. The survival rates of zebrafish infected with Ab-54149 cells (1~4 × 10⁷ CFU inoculum) were measured after intraperitoneal injection of PBS (blue line) or tailspike protein (TSP; 20 μg/fish, red line) 30 min after challenge. Zebrafish were also injected with 20 μg of TSP or 20 μL PBS alone in the absence of bacterial infection as controls (green line). The X-axis represents the post-time of infection and the Y-axis represents the survival percentage of the zebra fish. Statistical analysis was performed using the Kaplan–Meier method. Treatment with TSP (p < 0.05; log–rank test) significantly increased the survival of zebrafish infected with strain Ab-54149 after TSP treatment within the 4-day period.