Table 1.
Deiminated proteins in plasma EVs of reindeer (R. tarandus), identified by F95 enrichment and liquid chromatography with tandem mass spectrometry (LC–MS/MS) analysis. Proteins identified only in plasma EVs (and not in whole plasma) are highlighted in blue and with an asterix (*); uncharacterized hits with a secondary hit that was annotated are included and indicated in brackets. Protein ID, protein name, species hit with the Artiodactyla UniProt database, number of matches and total score are included in the table. For full detailed LC–MS/MS data for F95-enriched proteins, see Supplementary Table S1.
| Protein ID Protein Name |
Species Name Common Name |
Matches (Sequences) |
Total Score (p < 0.05) † |
|---|---|---|---|
|
A0A140T897_BOVIN
Albumin |
Bos taurus Cow |
2 (39) |
2322 |
|
L8ISP4_9CETA
Serum albumin |
Bos mutus Domestic Yak |
107 (37) |
2198 |
|
A0A4W2GW83_BOBOX
Uncharacterized protein (ALB protein) |
Bos indicus x Bos taurus Zebu x Cow |
105 (37) |
2072 |
|
A0A5N3XZ04_MUNRE
IF rod domain-containing protein |
Muntiacus reevesi Chinese muntjac |
44 (25) |
1128 |
|
*A0A212DF80_CEREH KRT5 |
Cervus elaphus hippelaphus European red deer |
44 (25) |
1275 |
|
A0A6J0WT46_ODOVR
Serum albumin |
Odocoileus virginianus texanus White-tailed deer |
52 (22) |
1177 |
|
A0A5N4DHW9_CAMDR
Keratin |
Camelus dromedarius Dromedary |
40 (21) |
1116 |
|
A0A4W2C021_BOBOX
Uncharacterized protein (collagen alpha-1 (I) chain) |
Bos indicus x Bos taurus Zebu x Cow |
34 (20) |
1080 |
|
A0A452FHU9_CAPHI
Uncharacterized protein (collagen type I alpha 1 chain) |
Capra hircus Goat |
34 (16) |
988 |
|
A0A5N3WTF4_MUNMU
Uncharacterized protein (collagen alpha-1(I) chain isoform X1) |
Muntiacus muntjac Barking deer |
23 (13) |
976 |
|
A0A5N4D320_CAMDR
Keratin |
Camelus dromedarius Dromedary |
37 (15) |
832 |
|
A0A6J0WBI9_ODOVR
Histidine-rich glycoprotein isoform X1 |
Odocoileus virginianus texanus White-tailed deer |
33 (14) |
806 |
|
*A0A212D793_CEREH
KRT19 |
Cervus elaphus hippelaphus European red deer |
32 (13) |
785 |
|
A0A287B5W2_PIG
Trypsinogen isoform X1 |
Sus scrofa Wild boar |
185 (157) |
765 |
|
A0A4W2D3K5_BOBOX
Keratin 75 |
Bos indicus x Bos taurus Zebu x Cow |
33 (13) |
729 |
|
A0A4W2DIS9_BOBOX
Keratin 75 |
Bos indicus x Bos taurus Zebu x Cow |
31 (15) |
724 |
|
A0A6B0R6W5_9CETA Uncharacterized protein (IF rod domain-containing; glial fibrillary acidic protein) |
Bos mutus Domestic yak |
25 (14) |
704 |
|
9XAP9_CAMFR
Keratin, type I cytoskeletal 14-like protein |
Camelus ferus Wild Bactrian camel |
24 (10) |
694 |
|
A0A452FN18_CAPHI
IF rod domain-containing protein |
Capra hircus Goat |
13 (9) |
676 |
|
A0A5N4DGN6_CAMDR
Keratin |
Camelus dromedarius Dromedary |
29 (14) |
670 |
|
A0A4W2IN22_BOBOX
IF rod domain-containing protein |
Bos indicus x Bos taurus Zebu x Cow |
25 (11) |
670 |
|
A0A6I9IRH0_VICPA keratin, type I cytoskeletal |
Vicugna pacos Alpaca |
10 (8) |
667 |
|
A0A5G2QXD3_PIG
IF rod domain-containing protein |
Sus scrofa Wild boar |
27 (18) |
655 |
|
*A0A3Q1LZN8_BOVIN
Collagen alpha-2(I) chain |
Bos taurus Cow |
26 (13) |
634 |
|
A0A287BLD2_PIG
Uncharacterized protein (collagen alpha-1(I) chain preproprotein; alpha 1 chain of type I collagen) |
Sus scrofa Wild boar |
19 (9) |
628 |
|
*A0A212D6S5_CEREH
KRT17 |
Cervus elaphus hippelaphus European red deer |
16 (11) |
580 |
|
A0A5N4DFY6_CAMDR
Keratin |
Camelus dromedarius Dromedary |
19 (12) |
550 |
|
A0A212CMY9_CEREH
Uncharacterized protein (immunoglobulin heavy constant; beta-2-microglobulin) |
Cervus elaphus hippelaphus European red deer |
10 (8) |
539 |
|
*A0A6J3QLJ4_TURTR
Collagen alpha-1(I) chain |
Tursiops truncates Common bottlenose dolphin |
9 (7) |
488 |
|
A0A2Y9SJP9_PHYMC
Keratin, type II cytoskeletal 6A |
Physeter macrocephalus Sperm Whale |
26 (10) |
469 |
|
A0A452EP10_CAPHI
IF rod domain-containing protein |
Capra hircus Goat |
9 (7) |
469 |
|
A0A6B0R542_9CETA
Uncharacterized protein (bradykinin; kininogen-1; kininogen-2) |
Bos mutus Wild yak |
16 (8) |
466 |
|
A0A5N4DG47_CAMDR
Keratin |
Camelus dromedarius Dromedary |
19 (9) |
463 |
|
A0A5N3WDS4_MUNMU
Bradykinin |
Muntiacus muntjac Barking deer |
10 (9) |
443 |
|
*A0A1S7J1Y9_PIG
Alpha2 chain of type I collagen |
Sus scrofa Wild boar |
17 (10) |
442 |
|
A0A5N4DFY1_CAMDR
Keratin |
Camelus dromedarius Dromedary |
13 (8) |
380 |
|
A0A383ZWF6_BALAS
Keratin, type II cytoskeletal 6A-like isoform X2 |
Balaenoptera acutorostrata scammoni Minke whale |
13 (8) |
360 |
|
W5Q4S0_SHEEP
Uncharacterized protein (collagen alpha-1(III) chain; collagen type III alpha 1 chain; fibrillar collagen NC1 domain-containing protein) |
Ovis aries Sheep |
7 (5) |
340 |
|
A0A2F0AVL6_ESCRO
Keratin, type II cytoskeletal 4 |
Eschrichtius robustus Gray whale |
12 (7) |
339 |
|
*A0A5N3W3N9_MUNRE
SH3 domain-containing protein |
Muntiacus reevesi Chinese muntjac |
8 (8) |
296 |
|
A0A340XVM8_LIPVE
Keratin, type I cytoskeletal 15 |
Lipotes vexillifer Baiji |
10 (6) |
294 |
|
A0A5N4CT25_CAMDR
Histone H4 |
Camelus dromedarius Dromedary |
5 (5) |
240 |
|
A0A5N3XAC4_MUNRE
Uncharacterized protein (Ig-like domain-containing protein) |
Muntiacus reevesi Chinese muntjac |
7 (3) |
239 |
|
A0A6J0ZDI0_ODOVR
Serotransferrin |
Odocoileus virginianus texanus White-tailed deer |
5 (5) |
234 |
|
*ACTB_BOSMU
Actin, cytoplasmic 1 |
Bos mutus grunniens Wild yak |
6 (5) |
232 |
|
A0A5N3WEA4_MUNMU
Beta-1 metal-binding globulin |
Muntiacus muntjac Barking deer |
5 (5) |
232 |
|
A0A6J0XRB4_ODOVR
Keratin, type II cytoskeletal 2 oral-like |
Odocoileus virginianus texanus White-tailed deer |
16 (6) |
228 |
|
A0A2C9F3E9_PIG
Junction plakoglobin |
Sus scrofa | 6 (1) |
228 |
|
A0A212DB90_CEREH
Ig-like domain-containing protein |
Cervus elaphus hippelaphus European red deer |
6 (3) |
225 |
|
*0A6I9IE32_VICPA
Collagen alpha-1(III) chain isoform X1 |
Vicugna pacos Alpaca |
6 (4) |
224 |
|
A0A212D5P4_CEREH
TAF domain-containing protein |
Cervus elaphus hippelaphus European red deer |
5 (5) |
222 |
|
A0A643C4S8_BALPH
Uncharacterized protein (IF rod domain-containing protein; KRT81; Keratin 85) |
Balaenoptera physalus Fin Whale |
10 (6) |
215 |
|
A0A5N3W8P2_MUNMU Uncharacterized protein (Ig-like domain-containing protein) |
Muntiacus muntjac Reeves’s muntjac |
7 (3) |
212 |
|
A0A212D7J2_CEREH
Fibrinogen beta chain |
Cervus elaphus hippelaphus European red deer |
3 (3) |
192 |
|
A0A287B7K6_PIG
IF rod domain-containing protein |
Sus scrofa Wild boar |
9 (6) |
186 |
|
A0A212DFA6_CEREH
IF rod domain-containing protein |
Cervus elaphus hippelaphus European red deer |
11 (5) |
184 |
|
A0A6J0XD83_ODOVR
Fibrinogen alpha chain |
Odocoileus virginianus texanus White-tailed deer |
5 (4) |
180 |
|
*A0A2Y9MPQ9_DELLE
Collagen alpha-1(III) chain |
Delphinapterus leucas Beluga whale |
5 (4) |
163 |
|
A0A452E8D3_CAPHI
Ig-like domain-containing protein |
Capra hircus Goat |
3 (2) |
149 |
|
W5P2K5_SHEEP
IF rod domain-containing protein |
Ovis aries Sheep |
7 (4) |
147 |
|
A0A5N3UHT3_MUNRE
Ig-like domain-containing protein |
Muntiacus reevesi Chinese muntjac |
2 (2) |
147 |
|
A2P2I1_SHEEP
VH region |
Ovis aries Sheep |
1 (1) |
131 |
|
*Q0VCX2|BIP_BOVIN
Endoplasmic reticulum chaperone BiP |
Bos taurus Cow |
2 (2) |
101 |
|
A0A212CAL2_CEREH
Elongation factor 1-alpha |
Cervus elaphus hippelaphus European red deer |
2 (2) |
100 |
|
A0A5N3UV43_MUNMU
IF rod domain-containing protein |
Muntiacus muntjac Barking deer |
5 (2) |
89 |
|
A0A3Q1LUE9_BOVIN
Ig-like domain-containing protein |
Bos taurus Cow |
1 (1) |
87 |
|
A0A6B9SDT6_BOVIN
Ig lamda chain variable region |
Bos taurus Cow |
1 (1) |
87 |
|
A0A212CSZ9_CEREH
Ig-like domain-containing protein |
Cervus elaphus hippelaphus European red deer |
2 (1) |
83 |
|
A0A2F0B9E6_ESCRO
Trypsin |
Eschrichtius robustus Gray whale |
2 (1) |
80 |
|
*A0A286ZKC5_PIG
HATPase_c domain-containing protein |
Sus scrofa Wild boar |
2 (0) |
76 |
|
A0A1L6BP13_BUBBU
Beta-casein |
Bubalus bubalis Water buffalo |
3 (2) |
75 |
|
A0A6B0S2F2_9CETA
Fibrinogen C-terminal domain-containing protein |
Bos mutus Wild yak |
3 (2) |
68 |
|
*P0C276|RL40_SHEEP
Ubiquitin-60S ribosomal protein L40 |
Ovis aries Sheep |
1 (1) |
67 |
|
*A0A2Y9SBW8_PHYMC
Histone H2B |
Physeter macrocephalus Sperm Whale |
2 (2) |
65 |
|
A0A6B0RTH8_9CETA
Uncharacterized protein (obscurin) |
Bos mutus Wild yak |
2 (2) |
63 |
|
A0A6J3S691_TURTR
Keratin, type II cytoskeletal 78 |
Tursiops truncates Common bottlenose dolphin |
2 (2) |
63 |
|
A0A383ZRF2_BALAS
Keratin, type I cytoskeletal 24 |
Balaenoptera acutorostrata scammony Minke whale |
1 (1) |
62 |
|
*A0A0C5AGQ3_BUBBU
Lysozyme |
Bubalus bubalis Water buffalo |
1 (1) |
61 |
|
A2P2I3_SHEEP
VH region |
Ovis aries Sheep |
1 (1) |
60 |
|
A0A075B7I6_PIG
Ig-like domain-containing protein |
Sus scrofa Wild boar |
1 (1) |
59 |
|
A0A0R4I993_SUSBA
Tubulin alpha chain |
Sus barbatus Bornean bearded pig |
1 (1) |
53 |
|
A0A5N4EAI9_CAMDR
Annexin |
Camelus dromedarius Dromedary |
2 (2) |
50 |
|
A0A2Y9EH04_PHYMC
Fer-1-like protein 4 |
Physeter macrocephalus Sperm Whale |
2 (2) |
50 |
|
A0A5J5N0U1_MUNRE Uncharacterized protein (small proline-rich protein 2I-like; Type II small proline-rich protein) |
Muntiacus reevesi Chinese muntjac |
1 (1) |
50 |
|
A0A6B0R269_9CETA
Ig-like domain-containing protein |
Bos mutus Wild yak |
2 (1) |
49 |
|
A0A452E907_CAPHI
Uncharacterized protein (skin-specific protein 32; Chromosome 3 C1orf68 homolog; Chromosome 1 open reading frame 68) |
Capra hircus Goat |
1 (1) |
48 |
|
A0A4W2E476_BOBOX
Ig-like domain-containing protein |
Bos indicus x Bos taurus Zebu x Cow |
1 (1) |
48 |
|
A0A212CS30_CEREH
Ig-like domain-containing protein |
Cervus elaphus hippelaphus European red deer |
2 (1) |
47 |
† Ions score is −10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 46 indicate identity or extensive homology (p < 0.05). Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.