Figure 3.

Morphology and morphometric analysis of LLCM-grown bone marrow derived macrophages in vitro. Isolated bone marrow cells were grown in cancer conditioned media (LLCM) or LLCM with 500 μM ascorbate (LLCM + Asc) for 7 days at ambient air (21% O₂) or subjected to hypoxia (1% O₂) on day 6 for ~18 h. (A) Images are representative using cells from one mouse (20×). Cell aspect ratio was determined by measuring the ratio of the longest cell axis to the shortest; higher ratios represents more spindled shaped cells. All conditions showed a mixed population of cells that ranged from spindled to spread out. (B) Cells grown with LLCM + Asc were less spindled overall compared to LLCM only, and statistical reduction in cell aspect ratio was observed at 21% O₂. Graphs depict data obtained from 3 independent experiments (median ± min/max). Two-tailed paired t-tests were performed, * denotes p < 0.05.