Figure 4.

Gene expression analysis of LLCM-grown bone marrow derived macrophages with or without ascorbate. Isolated bone marrow cells were grown with MCSF, LLCM or LLCM + Asc for 7 days at ambient air (21% O₂) or subjected to hypoxia on day 6 for ~18 h (1% O₂). Cells were harvested for gene expression analysis by qPCR. Gene expression levels were calculated relative to bone marrow cells cultured with MCSF at 21% O₂ and presented as fold change. Cells grown in LLCM showed altered gene expression levels compared to MCSF grown cells (left set of columns). Supplementation with ascorbate resulted in further significant gene expression changes (right set of columns). Oxygen tension also appears to alter expression of certain genes within each LLCM and LLCM + Asc cultured condition. Box and whiskers plot represent data from 5 independent experiments, box represents 25th to 75th percentile, whiskers represent min to max value and middle line represents median. Two-tailed paired t-tests were performed, * denotes p < 0.05 and ** denotes p < 0.01.