Figure 4.

(a) Competitive adhesion test of Caco-2 cells incubated with Lactobacillus and Salmonella. In Figure 4a, The target Salmonella are SC31, SC85, and SE05. In this experiment, Lactobacillus and Salmonella were added at the same time and incubated for 1 h, then the fluorescence intensity was measured. The stronger the fluorescence intensity was, the more red the color was. It showed that the number of Salmonella incubated on Caco-2 cells was more, while the fluorescence intensity was weak, the color was blue, indicating that the number of Salmonella incubated on Caco-2 cells was small. (b) Rejection and adhesion test of Caco-2 cells incubated by lactic acid bacteria first and then by Salmonella. In Figure 4b, the Lactobacillus isolated strains were added for incubation for 1 h, then the target Salmonella SC21, SC85 and SE05 were added for incubation for 1 h, and the results were measured by multi-functional enzyme labeling instrument. The stronger the fluorescence intensity was, the more red the color was. It showed that the number of Salmonella incubated on Caco-2 cells was more, while the fluorescence intensity was weak, the color was blue, indicating that the number of Salmonella incubated on Caco-2 cells was small. (c) Displacement adhesion test of Caco-2 cells incubated by Salmonella and then by lactic acid bacteria. In Figure 4c, the target Salmonella SC21, SC85 and SE05 were added to incubate for 1 h, and then the Lactobacillus isolate was added to incubate for 1 h, and then the results were measured by multi-functional enzyme labeling instrument. The stronger the fluorescence intensity was, the more red the color was. It showed that the number of Salmonella incubated on Caco-2 cells was more, while the fluorescence intensity was weak, the color was blue, indicating that the number of Salmonella incubated on Caco-2 cells was small.