Table 3.
Cross-reaction evaluation of Lyo-CRISPR SARS-CoV-2 kit with other respiratory pathogens.
| Pathogens | Source Cat. Number | Tested Concentration | Lyo-CRISPR SARS-CoV-2 | |||
|---|---|---|---|---|---|---|
| N Gene | N Gene Result | RNAseP | RNAseP Result | |||
| Human coronavirus 229E | 0810229CFHI | 105 TCID50/mL | 0/3 | Negative | 3/3 | Valid |
| Human coronavirus OC43 | 0810024CFHI | 105 TCID50/mL | 0/3 | Negative | 3/3 | Valid |
| Human coronavirus HKU1 | ATCC® VR-3262SD | 105 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Human coronavirus NL63 | ATCC® 3263SD | 105 copies/mL | 0/3 | Negative | 3/3 | Valid |
| SARS-CoV | NATSARS-ST | 105 TCID50/mL | 0/3 | Negative | 3/3 | Valid |
| MERS-CoV | NR-45843 | 105 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Respiratory syncytial virus | ATCC® VR-1580DQ | 105 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Influenza A H1N1 | ATCC® VR-95DQ | 105 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Influenza B (Yamagata Lineage) | ATCC® VR-1885DQ | 105 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Rhinovirus | NR-51453 | 105 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Mycobacterium tuberculosis | NR-14867 | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Streptococcus pyogenes | ATCC® 12344D-5 | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Streptococcus pneumoniae | ATCC® 700669D-5 | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Chlamydia pneumoniae | ATCC® VR-1360D | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Bordetella pertussis | ATCC® 9797D-5 | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Haemophilus influenzae | ATCC® 51907D-5 | 105 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Legionella pneumophila | ATCC® 33152D-5 | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Streptococcus salivarius | HM-121D | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Candida albicans | ATCC® 10231D-5 | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Pseudomonas aeruginosa | ATCC® 27853D-5 | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
| Staphylococcus epidermis | ATCC® 12228D-5 | 106 copies/mL | 0/3 | Negative | 3/3 | Valid |
Lyo: lyophilized; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2; CRISPR: clustered regularly interspaced short palindromic repeats; ATCC: American Type Culture Collection; TCID50: Tissue Culture Infectious Dose 50%.