Table 1.
Validation parameters for the determination of Aflatoxin M1 (AFM1) and Aflatoxin M2 (AFM2) in milk prior to the High-Performance Liquid Chromatography with Fluorescence Detector (HPLC-FL) method.
| Compound | Linearity R2 |
LOD 1 (ng kg−1) |
Intra-Day Recovery (%) RSD 2 (%) |
Inter-Day Recovery (%) RSD (%) |
CCα 3 (ng kg−1) |
CCβ 4 (ng kg−1) |
Error α | Error β | MRL 5 (ng kg−1) |
|---|---|---|---|---|---|---|---|---|---|
| AFM1 | 0.999 | 11.99 | 90–100 < 3.6 |
96–109 < 17 |
56.52 | 63.97 | 6.52 | 6.97 | 50 |
| AFM2 | 0.996 | 16.95 | 91–119 < 6.1 |
74–120 < 10.6 |
57.27 | 65.57 | 7.27 | 8.57 | − |
1 Limit Of Detection; 2 Relative Standard Deviation; 3 Decision Limit; 4 Detection Capability; 5 Maximum Residue Limits.