Table 2.
Results from the validation of three commercial Enzyme-Linked-Immunosorbent Assay (ELISA) kits of the detection and enumeration of AFM1 in milk samples.
| ELISA Kits | Within-Day Repeatability (n = 12) | Between-Day Precision (n = 9) Recovery (%RSD) |
LOD (ng kg−1) (Xmean + 3*SD) n = 20 |
LOQ (ng kg−1) (Xmean + 10*SD) n = 20 |
CCα (ng kg−1) |
CCβ ng kg−1) |
Error α | Error β | ||
|---|---|---|---|---|---|---|---|---|---|---|
| AFM1 True Concentration | Precision (%RSD) | Recovery | ||||||||
| Agraquant | AFM1 30 (ng kg−1) |
6.82% | 105% | 96−102% (6.33%) |
8.04 | 13.78 | 53.3 | 57.3 | 3.3 | 4.0 |
| AFM1 55 (ng kg−1) |
2.19% | 99% | 96–113% (13.99%) |
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| AFM1 80 (ng kg−1) |
5.37% | 86% | 78–86% (13.23%) |
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| AFM1 105 (ng kg−1) |
1.07% | 75% | -- | |||||||
| BioShield ES | AFM1 30 (ng kg−1) |
6.3% | 1091% | 107–112% (2.37%) |
7.94 | 14.41 | 53.9 | 58.1 | 3.9 | 4.2 |
| AFM1 55 (ng kg−1) |
2.45% | 1171% | 93–117% (12.65%) |
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| AFM1 80 (ng kg−1) |
2.43% | 95% | 80–95% (9.97%) |
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| AFM1105(ng kg−1) | 5.47% | 84% | -- | |||||||
| Ridascreen | AFM130 (ng kg−1) | 12.06% | 1074% | 107–127% (5.86%) |
9.44 | 15.70 | 54.0 | 58.8 | 4.0 | 4.8 |
| AFM1 55 (ng kg−1) | 1.65% | 117% | 117–124% (3.09%) |
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| AFM1 80 (ng kg−1) | 1.38% | 101% | 92–105% (12.89%) |
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| AFM1 105 (ng kg−1) | 3.31% | 82% | -- | |||||||