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. 2021 Mar 12;12(3):238. doi: 10.3390/insects12030238

Figure 4.

Figure 4

Transcript levels of CYP6B7 in midguts (A) and fat bodies (B) of fifth instar larvae of Helicoverpa armigera exposed to plant volatiles. Newly molted fifth instars were individually exposed to vola-tile 2-heptanone (Hept), cis-3-hexenyl acetate (Hylac), limonene (Limo) and nerolidol (Nero) at a dose of 1 µL per cup for 12, 24 or 48 h, respectively in a sealed cup. Then the midguts and fat bodies were dissected. Total RNA was pooled from midguts or fat bodies of 20 fifth instar H. ar-migera larvae exposed to different plant volatile for 12, 24 and 48 h. Control larvae (CK) were fed with diet without plant volatile. Real-time qRT-PCR analysis was conducted to determine the rel-ative transcript levels of the gene. Data shown are means ± SE derived from three biological re-peats (n = 3). Different letters above bars indicate significant differences (p < 0.05 using ANOVA followed by a Tukey test for post-hoc comparison).