Table 1.
Activities of glutathione-s-transferase (GST), carboxylesterase (CarE) and cytochrome P450 enzymes (P450s) in midguts and fatbodies of Helicoverpa armigera larvae exposed to volatile 2-heptanone (Hept), cis-3-hexenyl acetate (Hylac), limonene (Limo) and nerolidol (Nero) (mmol·min−1·mg·prot−1).
| Volatiles | GST | CarEs | P450s | |||
|---|---|---|---|---|---|---|
| Midgut | Fatbody | Midgut | Fatbody | Midgut | Fatbody | |
| CK | 3.56 ± 0.15 c | 1.78 ± 0.32 b | 28.55 ± 0.29 b | 6.23 ± 0.78 b | 0.26 ± 0.02 c | 0.21 ± 0.01 c |
| Hept | 5.12 ± 0.17 a | 2.75 ± 0.04 a | 37.45 ± 1.01 a | 9.74 ± 0.17 a | 0.52 ± 0.01 a | 0.79 ± 0.03 a |
| Hylac | 2.11 ± 0.19 d | 1.82 ± 0.05 b | 26.22 ± 1.29 b | 5.67 ± 0.15 b | 0.44 ± 0.02 a | 0.33 ± 0.01 b |
| Limo | 4.34 ± 0.24 b | 2.55 ± 0.04 a | 28.78 ± 0.59 b | 10.97 ± 0.64 a | 0.44 ± 0.03 a | 0.38 ± 0.01 b |
| Nero | 3.68 ± 0.12 bc | 1.85 ± 0.25 b | 28.00 ± 0.33 b | 5.36 ± 0.66 b | 0.35 ± 0.02 b | 0.32 ± 0.03 b |
Newly molted fifth instars were individually exposed to volatile 2-heptanone (Hept), cis-3-hexenyl acetate (Hylac), limonene (Limo) and nerolidol (Nero) at a dose of 1 µL per cup for 48 h in a sealed cup. Then midguts and fat bodies were dissected for enzyme assay. Control larvae (CK) were fed with diet without plant volatile. Data shown are means ± SE derived from three biological repeats (n = 3). Different letters above bars indicate significant differences (p < 0.05 using ANOVA followed by a Tukey test for post-hoc comparison).