Table 2.
Compounds | C | 1CA | 1S48 | 1S72 | 1CN | 10CA | 10S48 | 10S72 | 10CN |
---|---|---|---|---|---|---|---|---|---|
Residual sugars (g/L) | 0.13 ± 0.17 | 0.39 ± 0.29 | 1.22 ± 0.62 | 0.42 ± 0.57 | 29.61 ± 5.65 * | 0.42 ± 0.41 | 0.37 ± 0.22 | 0.88 ± 1.03 | 28.30 ± 6.29 * |
Ethanol production (%, v/v) | 13.06 ± 0.03 | 12.93 ± 0.06 | 12.58 ± 0.10 * | 12.57 ± 0.08 * | 11.04 ± 0.13 * | 12.77 ± 0.05 * | 12.41 ± 0.11 * | 12.29 ± 0.06 * | 10.91 ± 0.06 * |
Ethanol yield (g/g) | 0.47 ± 0 | 0.46 ± 0 | 0.45 ± 0 * | 0.45 ± 0 * | 0.46 ± 0.01 | 0.46 ± 0 | 0.45 ± 0 * | 0.44 ± 0 * | 0.45 ± 0.01 * |
Ethanol reduction (%, v/v) | 0 | 0.13 ± 0.06 | 0.48 ± 0.10 * | 0.49 ± 0.08 * | NC | 0.29 ± 0.05 * | 0.65 ± 0.11 * | 0.77 ± 0.06 * | NC |
Glycerol (g/L) | 6.57 ± 0.08 | 6.57 ± 0.22 | 5.41 ± 0.75 * | 5.74 ± 0.93 | 4.96 ± 0.41 * | 6.23 ± 0.11 | 6.70 ± 0.48 | 6.38 ± 0.18 | 5.44 ± 0.68 * |
Citric acid (g/L) | 0.13 ± 0 | 0.13 ± 0 | 0.11 ± 0.01 * | 0.12 ± 0.01 * | 0.12 ± 0 * | 0.12 ± 0.01 * | 0.12 ± 0.01 * | 0.11 ± 0 * | 0.11 ± 0 * |
Tartaric acid (g/L) | 2.79 ± 0.07 | 3.10 ± 0.14 | 3.41 ± 0.38 | 3.30 ± 0.31 | 3.38 ± 0.34 | 2.47 ± 1.10 | 3.23 ± 0.25 | 3.44 ± 0.14 | 3.38 ± 0.28 |
Malic acid (g/L) | 0.81 ± 0.13 | 0.63 ± 0.05 * | 0.50 ± 0.03 * | 0.45 ± 0.03 * | 0.39 ± 0.03 * | 0.61 ± 0.02 * | 0.54 ± 0.03 * | 0.51 ± 0.01 * | 0.44 ± 0.02 * |
Succinic acid (g/L) | 0.44 ± 0 | 0.43 ± 0.05 | 0.40 ± 0.05 | 0.36 ± 0.05 | 0.37 ± 0.02 | 0.44 ± 0.06 | 0.52 ± 0.03 | 0.52 ± 0.02 | 0.67 ± 0.05 * |
Lactic acid (g/L) | 0.20 ± 0.03 | 0.24 ± 0.02 | 0.24 ± 0.01 | 0.30 ± 0.10 * | 0.29 ± 0.01 * | 0.23 ± 0.05 | 0.30 ± 0 * | 0.33 ± 0.04 * | 0.25 ± 0.03 |
Acetic acid (g/L) | 0.10 ± 0.01 | 0.14 ± 0.03 | 0.29 ± 0.08 * | 0.36 ± 0.05 * | 0.32 ± 0.06 * | 0.22 ± 0.05 * | 0.23 ± 0.05 * | 0.22 ± 0.08 * | 0.21 ± 0.04 * |
Values are mean ± standard deviation of three independent replicates; The initial sugar concentration of synthetic must was 220 g/L; * means statistically significant differences from the control sample of C on the same row (LSD test, p < 0.05); NC means no calculate ethanol reduction due to stuck fermentation.