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. 2021 Mar 1;10(3):519. doi: 10.3390/cells10030519

Figure 5.

Figure 5

Analysis of miR-200c-3p and PD-L1 expression by RT-qPCR in SKOV3 cells treated with olaparib and irradiation. (A) (i): Non-irradiated: miR-200c-3p (upper-left graph) is inversely correlated with PD-L1 expression (bottom-left graph) in both DMSO and 1.5 μM olaparib-treated SKOV3 cells, (A) (ii): Irradiated: In DMSO-treated SKOV3 cells, miR-200c-3p and PD-L1 are inversely correlated. Co-treatment with 1.5 μM olaparib and irradiation shows an increase of PD-L1, regardless of high miR-200c expression. (B) (i): Non-irradiated: At the higher dose of olaparib, 5 μM, PD-L1 is inversely correlated to miR-200c-3p. (B) (ii): Irradiated: PD-L1 mRNA is induced in untreated and olaparib 5 μM-treated cells. Experiments were repeated three times and in technical triplicates. p-values were calculated by Dunett’s multiple comparisons: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant.