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Gating strategy used to select the population of interest using a FACS Verse cytometer. (a) Nasal epithelial cells were selected by side scatter characteristic (SSC) and forward scatter characteristic (FSC) density plots. (b) A gate was applied to identify specific populations of individual cells. Each dot represents one nasal epithelial cell that passed through the cytometer laser. (c) Dead cells were identified and excluded from further analysis using either DAPI or PI.
