Figure 1.

Effect of the soluble fraction of bacterial lysates from VSL#3 and Vivomixx formulations on colonic intestinal fibroblast cells (CCD-18Co) cell number. (A) CCD-18Co cells were starved for 24 h and then incubated with TGF-β1 (10 ng/mL) for 48 h in the presence or absence of VSL#3- or Vivomixx-derived fractions (50 µg/mL), in serum-free medium. The values represent the cell number expressed as mean ± SEM of three independent experiments in duplicate. For comparative analysis of data groups, a one-way analysis of variance (ANOVA) with post hoc Bonferroni test was used. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Representative phase-contrast microscopic images showing CCD-18Co fibroblasts treated as described in (A).