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. 2021 Mar 9;13(3):882. doi: 10.3390/nu13030882

Figure 2.

Figure 2

Influence of the soluble fraction from VSL#3 and Vivomixx lysates on TGB-β1-induced collagen I expression in CCD-18Co cells. (A) Immunoblotting assay for collagen I was performed on CCD-18Co cells incubated for 48 h with TGF-β1 (10 ng/mL) in the presence or absence of VSL#3- or Vivomixx-derived fraction (50 µg/mL). Following densitometric analysis, obtained values were normalized vs. GAPDH. Data are from three independent experiments in duplicate, and values are expressed as mean ± SEM. For comparative analysis of data, a one-way analysis of variance (ANOVA) with post hoc Bonferroni test was used. ** p < 0.01, *** p < 0.001. A representative immunoblot of collagen I and GAPDH is also shown. (B) Representative immunofluorescence images of CCD-18Co cells treated as described in (A) and stained with anti-collagen I antibody (green) and with TRITC-phalloidin (red) to reveal F-actin. Nuclei were counterstained with DAPI (blue) (magnification 40×). The images are representative of three independent experiments in duplicate.