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. 2021 Mar 13;9(3):297. doi: 10.3390/biomedicines9030297

Figure 2.

Figure 2

UA induced cell cycle arrest and apoptosis in NSCLC cells. (A) The western blotting for CDK4, cyclin D1, cyclin E1, p21 (CDKN1A), and p27 (CDKN1B) in A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. β-actin was used as the housekeeping gene. (B) RT-qPCR analysis of the expression of cell cycle checkpoint genes CCND1, CCNE1, CDK4, CDKN1A, and CDKN1B in A549 and H460 cells after treatment with 10 and 20 µM UA, respectively, for 24 h. The Cp values were normalized to GAPDH mRNA. Controls are set to 100. (C) Flow cytometry analysis with PI staining of the cell cycle distribution in A549 and H460 cells after treatment with 20 µM UA for 24 h, with a graphical representation of G0/G1 arrest by UA in NSCLC cells. (D) Flow cytometry analysis using annexin V and PI staining in A549 and H460 cells after treatment with 20 µM UA for 24 h. *** p < 0.001 (ANOVA test), and # p < 0.001 vs. control.