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. 2021 Mar 13;9(3):297. doi: 10.3390/biomedicines9030297

Figure 3.

Figure 3

UA inhibited NSCLC cell angiogenesis, invasion, and migration. (A) The inhibition of angiogenesis by 10 and 20 µM UA during the in vitro angiogenesis assay of A549 and H460 cells, respectively. (B) Graphical representation of the in vitro angiogenesis assay showing the relative inhibition of tube formation. (C) Western blotting of A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h for phospho-STAT3, STAT3, and VEGF. The relative levels of the proteins were determined by densitometry and normalized to that of β-actin, which was set to 100. The data were confirmed by repeating the experiment three times. (D) The Matrigel invasion assay showing the inhibition of invasion in A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 24 h. Scale bars: 100 µm. (E) The wound healing assay demonstrated the inhibition of migration in A549 and H460 cells treated with 10 and 20 µM UA, respectively, for 0 and 24 h. Scale bars: 50 µm. Statistical analysis was conducted using ANOVA test; *** p < 0.001; # p < 0.001 vs. control.