UEv protein profiles. NuPAGE® 4–12% electrophoresis and Sypro Ruby protein gel staining. (a) Patients affected by INS: steroid-sensitive (SSSN), steroid-dependent (SDSN), steroid-resistant (SRSN); three representative cases for each patient group are shown. (b) UEv protein profile reproducibility: UEv isolated from the same patients at different time of collection (I, first collection; II, secondo collection; III third collection). (c) UEv protein profile of healthy controls (HC) and patients affected by hereditary tubulopathies (GS, Gitelman syndrome; BS1, Bartter syndrome type 1; BS2, Bartter syndrome type 2): two representative cases for each patient group are shown [17]. UEv protein profiles correspond to 3 mL of starting urine.