Figure 1.

Phyllostachys pubescens leaves extract (PPE) suppressed human SRD5A2 promoter activity and mRNA expression. (A) Concentration-response effects of PPE on human SRD5A2 promoter activity. BPH-1 cells were transfected with the luciferase reporter plasmid vectors comprising the human SRD5A2 promoter sequence. Cells were treated with the indicated concentrations of PPE for 24 h and luciferase activity was measured by a luminometer. (B) mRNA levels of SRD5A2 were measured by Q-PCR. (C) Cytotoxicity effects of PPE. All data are shown as mean ± S.E.M. of three independent experiments with triplicate wells. * p < 0.05, ** p < 0.01, and *** p < 0.001, compared with CON.