Table 1.
The primers’ sequences used in the Real-Time PCR.
| Gene | Primer | Nucleotide Sequence 5′-3′ | PCR Product [bp] |
|---|---|---|---|
| DNMT1 | forward reverse |
GTGGAAGCCGGCAAAGC TCCCACTCGAGCCTTCCATA |
125 |
| DNMT3A | forward reverse |
AAGGAGGAGCGCCAAGAG ATCACCGCAGGGTCCTTT |
113 |
| EHMT1 | forward reverse |
CCTCGACTCGGAAAAACCCAA AGAGCGCTTATTCTGGTGCT |
148 |
| EHMT2 | forward reverse |
CATCGATCGCAACATCACCC GAGCAATCGCCCATCCTTGT |
121 |
| HDAC3 | forward reverse |
TGACTCTCTGGGCTGTGATCG CATATTCAACGCATTCCCCATGC |
74 |
| HDAC5 | forward reverse |
CTTAGCAAGTGCGAGCGGAT TGTCTAGCTTCTGCCGGTTG |
121 |
| TET3 | forward reverse |
CACTAGCTGTACCAACCGCC CAGCCTTTATTTCCACCTCCTTGA |
104 |
| House keeping genes | |||
| GAPDH | forward reverse |
AGCCACATCGCTCAGACAC GCCCAATACGACCAAATCC |
66 |
| RPL0 | forward reverse |
TCTACAACCCTGAAGTGCTTGAT CAATCTGCAGACAGACACTGG |
96 |
| RPL13 | forward reverse |
CAAGCGGATGAACACCAAC TGTGGGGCAGCATACCTC |
95 |