Figure 1.

Zinc aspartate suppressed proliferation in freshly stimulated and pre-activated T cells. Human resting T cells were freshly stimulated (A) or pre-activated for 48 h (B) with anti-CD3/CD28 antibodies and cultured with increasing concentrations of zinc aspartate. Kinetic measures of the cluster area per well were recorded by the IncuCyte S3 imaging system at 3 h intervals for 72 h. Data are presented as the mean + standard error of the mean (SEM) from three independent experiments. (* p < 0.05, ** p < 0.01, *** p < 0.001). Representative images of proliferating cell clusters from freshly stimulated (C) or pre-activated (D) T cells treated with the indicated concentration of zinc aspartate are shown. Scale bar, 400 µM.