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. 2021 Mar 1;9(3):246. doi: 10.3390/biomedicines9030246

Figure 4.

Figure 4

Zinc aspartate impaired membrane integrity of freshly stimulated and pre-activated T cells. Human resting T cells were freshly stimulated (A) or pre-activated for 48 h (B) with anti-CD3/CD28 antibodies and cultured with increasing concentrations of zinc aspartate. For kinetic analyses, T cells were cultured with increasing concentrations of zinc aspartate in the presence of CytoTox Red reagent. Kinetic measures of the number of CytoTox Red positive cells were recorded by the IncuCyte S3 imaging system at 3 h intervals for 72 h. Data are presented as the mean + SEM from three independent experiments (** p < 0.01, *** p < 0.001).