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. 2021 Mar 12;22(6):2900. doi: 10.3390/ijms22062900

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phenotype of HSF30-2 silencing in tobacco under heat stress. (A) Phenotype of HSF30-2-silencing in tobacco by virus-induced gene silencing (VIGS) before and after heat shock. TRV-HSF30-2 means the silencing of the CvHSF30-2 homologous gene in tobacco. TRV-HSF30-2① and TRV-HSF30-2② represent two different HSF30-2-silencing lines. (B) Silencing effect of TRV-HSF30-2 plants based on qPCR. (C,D) DAB and NBT staining of HSF30-2-silenced tobacco after heat treatment. (E,F) DAB and NBT staining quantified using the ImageJ2x program. The unit on the x-axis is the average grey value. The staining intensity was quantified using the ImageJ2x program. The image (inverse phase) was changed into an 8-bit grey image, and the average grey values from the upper, middle and lower areas of root tips calculated by ImageJ2x reflect the staining intensity. Error bars represent the standard error of three biological replicates. * p < 0.05; ** p < 0.01. TRV: tobacco rattle virus; CK: control check (normal temperature).