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Silencing of CvHSFB2a in Clematis. (A) Phenotype of CvHSFB2a-silenced Clematis under heat treatment. (B) DAB and NBT staining of TRV-GFP and TRV-CvHSFB2a Clematis before and after heat treatment. (C) Silencing effect of CvHSFB2a. (D) The expression of CvHSF30-2 in TRV-CvHSFB2a plants. (E,F) DAB and NBT staining as quantified using the ImageJ2x program. Error bars represent the standard error of three biological replicates. * p < 0.05; ** p < 0.01.
