Figure 3.

BRAF V600E serial dilutions in a constant background of BRAF WT. Log of absolute copy number of BRAF V600E (blue) and BRAF WT (green) are plotted against log mutant frequency. Data points are plotted as mean +/− SD. Serial dilutions of Yumel 0106 gDNA were prepared in a constant background of HBMVEC gDNA from 10% to 0% mutant allele frequency to determine the limit of detection and limit of blank of the ddPCR assay. The limit of blank (LOB), the highest apparent BRAF V600E concentration expected to be found in a sample without mutant input, and limit of detection (LOD), the concentration of BRAF V600E that can be reliably distinguished from background, are denoted by the dotted lines.