Immunogenicity results from a dose-ranging study in mice with S. Typhimurium GMMA stressed in harsh temperature conditions: (A) anti-OAg IgG by ELISA, (B) anti-GMMA protein IgG by ELISA and (C) SBA against S. Typhimurium wild-type strain induced by control GMMA formulated on Alhydrogel and GMMA stressed at 100 °C for 40 min before (in the legend, DS, drug substance, 100 °C 40′) or after (DP, drug product, 100 °C 40′) formulation on Alhydrogel. Single mice EU/mL in ELISA and SBA titers (dots) are reported together with geomean for each group (bars) and 95% confidence interval. Parallel line analysis showed that Y-intercepts of the dose–response curves of the three different formulations were not significantly different among each other in the case of anti-OAg (D), anti-GMMA protein ELISA (E) and SBA titers (F), being their p-values above the specified alpha of 0.05.