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. 2021 Mar 7;10(3):401. doi: 10.3390/antiox10030401

Figure 5.

Figure 5

Impact of the CXDXXC motif on PFK activity and oxidation sensitivity. Single (a) and double (b) Cys to serine mutations were introduced in A. thaliana AtPFK5 proteins with CXDXXC motif. The variants were transiently overexpressed in tobacco leaves. Extraction buffer contained 5 mM β-mercaptoethanol (β-ME). After determining the initial activity, samples were oxidized with 2.5 mM sodium tetrathionate (TT) for 20 min and afterwards re-reduced with 5 mM dithiothreitol (DTT). Upper case letters show significant differences for each isoform to buffer control, lower case letters indicate significant differences among treatments for each protein at p < 0.05 (ANOVA, Post hoc Tukey test, n = 3). (c) Detail of the protein alignment of the CXDXXC motif in PFKs from A. thaliana and O. sativa. The two mutated Cys are marked with an arrow.