Fluctuation of simulated ambient temperatures throughout the experiment (A) and throughout the day (B). Turquoise, yellow, and red bars represent familiarisation day, pre/post, and during heat-wave periods, respectively. Dark and light colours correspond to the simulated ambient temperatures during work and rest, respectively. Lines represent the two levels (work and rest) of heat stress during (red) and pre/post (yellow) the simulated heat-wave. Work-shifts were scheduled between 08:40 and 18:00. A strict time-framed (wake up: 07:00, breakfast: 08:00, work: 08:40–12:00, lunch: 12:00, work: 12:40–18:00, dinner: 18:20, free time: 19:00–23:00, and sleep: 23:00) protocol was followed. The time periods spent on passive heat exposure (i.e., sitting in the workplace without having work to perform; duration, 5:20), simulated assembly line tasks (SALT; 2 × 60 min), and stepping sessions (2 × 40 min) are distinguished by different shades of grey. The remaining time was dedicated for meals, free time, and sleep in controlled environmental conditions.