Figure 2.

Time–kill curves of B. subtilis cultures expressed as Log₁₀ of CFU counts after treatment with different antimicrobial substances (celastrol 3 µg/mL; pristimerin 10 µg/mL; ciprofloxacin 1.2 µg/mL; rifampicin 0.15 µg/mL; tetracycline 7.5 µg/mL; and penicillin 9 µg/mL) added in lag phase of growth (A) and log phase after three hours of preincubation (B). Cultures without drugs and with the maximum proportion of DMSO were used as controls. Error bars express SD with n = 3.