Skip to main content
. 2021 Mar 12;13(3):470. doi: 10.3390/v13030470

Figure 2.

Figure 2

Primary FIV vaccination also induced a higher anti-gp40 antibody response than annual FIV re-vaccination. Anti-gp40 antibodies increased significantly over time in both primary and annual vaccination groups (Primary: p = 0.024; Annual: p = 0.0022; blue and red bars below the box plots, respectively; mixed-effects analysis). Anti-gp40 antibodies differed significantly over time between groups (interaction, p < 0.0001) and at individual time points following vaccination (day 14 *: p < 0.001; day 28 †: p = 0.012, where the black bars joining the box plots indicate the analysis of the comparative time points), with primary vaccination inducing higher levels of antibodies compared to annual vaccination (mixed-effects analysis). Samples from days 0, 14 *, and 28 † post-final vaccination were compared (Primary: days 0, 70 * and 84 †; Annual: days 0, 14 *, and 28 †). NF = (sample OD)/(2 × negative control OD). OD = optical density. * = p < 0.05, *** = p < 0.001.