Reagent Type (Species) or Resource	Designation	Source or Reference	Identifiers	Additional Information
Cell line (H. sapiens)	HeLa	ATCC (Manassas, VA, USA)	CCL-2	Maintained in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin and 1% L-Glutamine
Antibody	Rabbit anti-Aurora A	Abnova (Taipei, Taiwan)	PAB0359	1:1000
Antibody	Rabbit anti-Aurora-A(T288)	Abnova (Taipei, Taiwan)	MAB7299	1:1000
Antibody	Rabbit anti-AIBp	Abcam (Cambridge, MA, USA)	ab122014	1:500
Antibody	Rabbit anti-Plk1	Cell signaling (Beverly, MA, USA)	s4513	1:1000
Antibody	Rabbit anti-Plk1(Thr210)	Cell signaling (Beverly, MA, USA)	S5472	1:1000
Antibody	Mouse anti-Drp1	BD (BD Biosciences, San Jose, CA, USA)	611112	1:1000
Antibody	Mouse anti-Drp1 (Ser 616)	Cell signaling (Beverly, MA, USA)	S3455	1:1000
Antibody	Mouse anti-Drp1 (Ser 637)	Cell signaling (Beverly, MA, USA)	S4867	1:1000
Antibody	Rabbit anti-Opa1	Cell signaling (Beverly, MA, USA)	S67589	1:1000
Antibody	Rabbit anti-Mfn1	Cell signaling (Beverly, MA, USA)	S14739	1:1000
Antibody	Rabbit anti-Mfn2	Cell signaling (Beverly, MA, USA)	S9482	1:1000
Antibody	Rabbit anti-cdc (Cdk1)	Cell signaling (Beverly, MA, USA)	S28439	1:1000
Antibody	Rabbit anti-cdc2 (Thr14)	Cell signaling (Beverly, MA, USA)	S2543	1:1000
Antibody	Rabbit anti-Cyclin B	Cell signaling (Beverly, MA, USA)	S12231	1:1000
Antibody	Rabbit anti-Cyclin B (S133)	Cell signaling (Beverly, MA, USA)	S4133	1:1000
Antibody	Rabbit anti-VDAC1	Invitrogen (Life Technologies, CA, USA)	PA1-954A	1:1000
Antibody	Rabbit anti-ch-TOG (CKAP5)	GeneTex (Irvine, CA, USA)	GTX30693	1:1000
Antibody	Rabbit anti-TACC3	Santa Cruz (Santa Cruz, CA, USA)	SC-22773	1:500
Antibody	Rabbit anti-TACC3 (Ser 558)	Cell signaling (Beverly, MA, USA)	S8842	1:1000
Antibody	Mouse anti-P62	Abcam (Cambridge, MA, USA)	Ab56416	1:2000
Antibody	Rabbit anti-LC3B	Cell signaling (Beverly, MA, USA)	S3868	1:1000
Antibody	Mouse anti-GAPDH	Arigo (Hsinchu, Taiwan)	ARG10112	1:5000
Antibody	Rabbit anti-PINK1	Elabscience (Houston, TX, USA)	E-AB-17371	1:1000
Antibody	Rabbit anti-PRKN	Elabscience (Houston, TX, USA)	E-AB-63490	1:1000
Antibody	Mouse anti-α-tubulin	Sigma-Aldrich (St. Louis, MO, USA)	T5168	1:5000
Antibody	Rabbit anti-γ-tubulin	Sigma-Aldrich (St. Louis, MO, USA)	T5192	1:5000
Antibody	Total OXPHOS rodent WB antibody cocktail (Mouse)	Abcam (Cambridge, MA, USA)	ab122014	1:1000
Commercial assay or kit	Mitochondria Isolation Kit	Thermo Fisher (Waltham, MA, USA)	89874
Commercial assay or kit	TMRM Reagent	Invitrogen (Life Technologies, CA, USA)	I34361	Mitochondrial membrane potential indicator
Commercial assay or kit	CellROX™ Green Reagent	Invitrogen (Life Technologies, CA, USA)	C10444	Oxidative stress detection
Commercial assay or kit	ATP Assay Kit	Sigma-Aldrich (St. Louis, MO, USA)	MAK190	ATP Colorimetric/Fluorometric
Commercial assay or kit	Cell counting Kit-8	Sigma-Aldrich (St. Louis, MO, USA)	96992	Proliferation and cytotoxicity assays
Commercial assay or kit	MitoTracker	Invitrogen (Life Technologies, CA, USA)	M7512