Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Comparison of the initial GMUCT2.0 and the improved protocol. The initial protocol takes 3 days and includes library gel size selection and many precipitations steps (highlighted in orange). To simplify GMUCT2.0 protocol, we adjusted volume reactions to limit precipitation steps, used a more efficient reverse transcriptase and replaced library gel size selection by a library beads clean-up (highlighted in red). Approximative duration time is indicated for each step.
