Table 1.

Recommended amplification according to the amount of total RNA used as a starting point. After amplification, a library molarity between 10 and 15 nM is expected. This molarity is adequate for following sequencing steps.

Starting Material (Total RNA)	Recommended PCR Cycles	Yield ng/μl	Library Molarity (nM)
50 μg	10–11	3–4	10–15
5 μg	15–16	3–4	10–15
0.5 μg	16–17	3–4	10–15