Table 1.
Permutation analyses of variance (PERMANOVAs) on the effect of application of three doses of zinc (Zn) (0.1, 1, and 10 mg Zn plant−1) and plant compartment (shoot and root) on the expression of seven MsZIP genes and separately on the expression of other five genes (MsZIF1, MsNAS1, MsHMA4, MsYSL1, and MsMTP1). A PERMANOVA was also performed on the response of all the genes. The analysis of homogeneity of multivariate dispersion (PERMDISP) was also performed. The studied plant was alfalfa (Medicago sativa L.). The analysis also included no-Zn addition control.
| Response Variables | Explanatory Variables |
Zn Application (Zn) | Plant Compartment (Comp) | Zn x Comp | Residual |
|---|---|---|---|---|---|
| ZIP genes | Pseudo F | 5.56 | 8.16 | 1.76 | |
| P(perm) | 0.002 | 0.001 | 0.082 | ||
| Explained variance (%) | 29.1 | 22.9 | 9.7 | 38.3 | |
| PERMDISP P(perm) | 0.412 | 0.852 | |||
| Other genes | Pseudo F | 3.06 | 5.59 | 1.76 | |
| P(perm) | 0.007 | 0.015 | 0.1 | ||
| Explained variance (%) | 17.3 | 19.35 | 12.78 | ||
| PERMDISP P(perm) | 0.412 | 0.852 | |||
| All genes | Pseudo F | 4.27 | 10.49 | 3.41 | |
| P(perm) | 0.001 | 0.001 | 0.003 | ||
| Explained variance (%) | 17.3 | 25.2 | 25.6 | 31.9 | |
| PERMDISP P(perm) | 0.152 | 0.030 |