Skip to main content
. 2021 Mar 4;12(3):363. doi: 10.3390/genes12030363

Figure 3.

Figure 3

Osa limits dpp transcription in ECs for germline differentiation control. (A) Quantitative PCR results for the purified ECs showing that mRNA levels for the annotated dpp transcripts were upregulated in osa KD ECs. (B,C,D) Germaria stained for α-spectrin (red), Armadillo (red), and β-gal (green). dpp transcription was monitored by dpp2.0-lacZ (detected by β-gal). CpCs are indicated by white dashed circles. In the control germarium (B), dpp2.0-lacZ was expressed in CpCs. In the osa RNAi-1 (C) and osa RNAi-2 (D) germaria, dpp2.0-lacZ was also expressed in ECs (indicated by white arrows). (E–K) Germaria stained for α-spectrin (red), Armadillo (red), and DAPI (blue). (H) The control germaria exhibiting a normal number of CBs. The osa RNAi-1 (E) and osa RNAi-1+ mcherry RNAi (I) germaria exhibiting UGC accumulation. The introduction of one copy of the dpp allele (F,G) into the background of c587 > osa RNAi-1 or dpp RNAi (J,K) into the background of c587>osa RNAi-1 relieved the UGC accumulation phenotype. c587>osa RNAi-2 provided similar phenotypes (not shown). (L,M) Graphs showing quantification of the UGC number per germarium. Error bars are presented as the Mean ± SD. Several compressed z-sections are shown (B–K). The scale bar is shown in (B).