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. 2021 Mar 4;12(3):363. doi: 10.3390/genes12030363

Figure 5.

Figure 5

Osa regulates dpp partially through Engrailed. (A–G) Germaria stained for α-spectrin (red), Armadillo (red), and DAPI (blue). The osa RNAi-1 (A) and osa RNAi-1+mcherry RNAi (E) germaria exhibited UGC accumulation. (D) The mcherry RNAi germaria exhibited a normal number of CBs. Introduction of one copy of an en allele (B,C) or en RNAi (F,G) significantly relieved the germ cell differentiation defect. (H,I) Graphs show the quantification of UGC number per germarium. Error bars are presented as the Mean ± SD. (J–P) Germaria stained for α-spectrin (red), Armadillo (red), and β-gal (green). dpp transcription was monitored by dpp2.0-lacZ (detected by β-gal). CpCs are indicated by white dashed circles. In the control germarium (J), dpp2.0-lacZ was expressed in CpCs. In osa RNAi-1 (K) and osa RNAi-2 (N) germaria, dpp2.0-lacZ was ectopically expressed in ECs (indicated by white arrows) in addition to CpCs. The introduction of en RNAi-1 (L,O) or en RNAi-2 (M,P) reduced the ectopic dpp2.0-lacZ in ECs. (Q) A graph showing the quantification of dpp2.0-lacZ-positive ECs for each germarium. Error bars are presented as the Mean ± SD. Several compressed z-sections are shown in (A–G,J–P). The scale bar is shown in (A).