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. 2021 Mar 11;14(3):253. doi: 10.3390/ph14030253

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lipid accumulation in 3T3-L1 adipocytes after treated with essential oil, extracts and positive controls (adrenaline 0.1 mM or 18.3 µg/mL and caffeine 1 mM or 194.2 µg/mL); (A) in the preventive experiments where the pre-adipocytes were treated with the samples during the differentiation on days 3, 5 and 7, and (B) in the treatment experiments where the samples were added after the pre-adipocytes were differentiated to adipocytes (on day 9) and incubated for one day. The lipid accumulation was measured by Oil Red O assay, and the results are expressed as the mean ± SEM of triplicate tests. Data expressed in percentage in comparison with control. One-way ANOVA showed significant value, * p < 0.05 as compared to control.

Lipid accumulation in 3T3-L1 adipocytes after treated with essential oil, extracts and positive controls (adrenaline 0.1 mM or 18.3 µg/mL and caffeine 1 mM or 194.2 µg/mL); (A) in the preventive experiments where the pre-adipocytes were treated with the samples during the differentiation on days 3, 5 and 7, and (B) in the treatment experiments where the samples were added after the pre-adipocytes were differentiated to adipocytes (on day 9) and incubated for one day. The lipid accumulation was measured by Oil Red O assay, and the results are expressed as the mean ± SEM of triplicate tests. Data expressed in percentage in comparison with control. One-way ANOVA showed significant value, * p < 0.05 as compared to control.