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. 2021 Mar 15;9(3):302. doi: 10.3390/biomedicines9030302

Figure 3.

Figure 3

Explant-induced M2-like Mφ polarisation is reversed upon LPS and IFNγ treatment. (A) To test the plasticity of the explant-induced M2-like phenotype, explant-conditioned Mφ co-cultures were treated in parallel with or without M1-promoting cytokines (20 ng/mL IFNγ and 10 pg/mL LPS) for 48 h. Each symbol represents an individual patient. (B) Pie charts representing the proportion of CD14+ cells exhibiting an M1-, M2-, MU-, and MT-like phenotype following 48 h ex vivo culture with explants in the absence (left) and presence (right) of M1-promoting cytokines. MT, Transitional myeloid cells; MU, Unpolarised myeloid cells. The p values were obtained from one-way ANOVA followed by post hoc Tukey test. Statistically significant differences were observed compared to the M2 control (M1-like Mφs) or M1 control (M2-like Mφs). Data were obtained from 5 independent experiments using patient blood and tissue and graphs show means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.