Figure 1.

Identification of the crassulacean acid metabolism (CAM)-isoform of phosphoenolpyruvate carboxylases (PEPC) in Agave americana. (a) Phylogenetic relationships of PEPCs in A. americana and plant-type PEPCs from other monocot plants. Bootstrap values are shown at nodes. The sources for the PEPC sequences (as well as species abbreviations, etc.) are provided in ‘Materials and Methods’. (b) Diel transcript expression of the PEPC genes in A. americana. The Z-score is defined as (x_i − μ_i)/σ_i, where x_i is the FPKM of gene i, μ_i is the mean and σ_i is the standard deviation of all 15 columns. White and black bars indicate daytime (12 h) and nighttime (12 h), respectively. Mleaf: mature leaf; Yleaf: young leaf.