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. 2021 Mar 6;10(3):582. doi: 10.3390/cells10030582

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Changes in proline biosynthesis in AaPEPC1-overexpressing tobacco plants. (a) Proline content in the leaves of transgenic plants expressing AaPEPC1 (OE1 and OE2) as well as empty vector (EV), and wild-type plants (WT). (b) and (c) Relative expression level of the proline biosynthesis genes pyrroline-5-carboxylate synthase (P5CS) and pyrroline-5-carboxylate reductase (P5CR) in the AaPEPC1-overexpressing tobacco plants. Values represent means ± SD (n = 3). * and ** indicate significant difference from that of WT at p < 0.05 and p < 0.01, respectively, by one-way ANOVA analysis with post-hoc Tukey HSD.