Figure 2.

Human PR increased ALDH activity and CD133 expression in OvCa cells. (A–D) OvCa cells treated for 5 days with or without 10% PR were dissociated into single-cell suspensions by trypsinization, and then ALDH activity and CD133 expression were evaluated by means of flow cytometry. Results represent the percentage of ALDH-positive (A) and CD133-positive (C) cells. Data are expressed as mean ± SEM of three replicates with three different PR pools. * p < 0.01 (untreated vs. PR treated cells). (B) Representative flow cytometric dot-plots showing ALDH-positive cells. The ALDH inhibitor diethylaminobenzaldehyde (DEAB) was used as a negative control. (D) Representative flow cytometric histograms showing CD133 expression.