Figure 12.

Viability of endothelial cells (BALB-5206 ad HUVEC) subjected to the bioprinting process with different pressures and nozzles with an internal diameter of 0.6 (Figure 8a,c) and 0.2 mm (Figure 8b,d). As a carrier material, 3% (w/v) alginate was used, and the vitality of the islets was assessed based on FDA/PI staining and determined in accordance with Equation (7). Our control consisted of cells right after the trypsinization process. The p-value for each pressure was calculated in comparison to the control group. In BALB 5206, p-value was determined using Fisher’s method, and statistical significance (p < 0.05) was observed in each pressure of 0.2 mm nozzle and in almost even pressure of 0.6 mm nozzle (excluding 15 kPa). In HUVEC, p-value was determined using Fisher’s method, and statistical significance (p < 0.05) was observed in each pressure of 0.2 mm nozzle and only in 100 kPa of 0.6 mm nozzle. N = 3.