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. 2021 Mar 10;22(6):2809. doi: 10.3390/ijms22062809

Figure 3.

Figure 3

Danegaptide reduces TGFβ1-induced mRNA changes in cell cycle and reno-protective markers. Primary hPTECs were cultured in low glucose (5 mM) ± TGFβ1 (10 ng/mL) ± danegaptide (100 nM) for 12 h. In TGFβ1-treated cells, qPCR analysis demonstrated an increase in p16, p21 and cyclin D1 mRNA and a significant reduction in Klotho mRNA as compared to controls. Danegaptide (100 nM) partly negated the increase in p16, p21 and cyclin D1 and reversed the TGFβ1-evoked change in Klotho. Results are representative of 3 separate experiments and presented as the mean ± SEM (n = 3), with key significances indicated (** p < 0.01, *** p < 0.001; one-way ANOVA and Tukey’s post-test).